Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 24667-1-AP (TBC1D4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded human colon slide using 24667-1-AP (TBC1D4 Antibody) at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24667-1-AP (TBC1D4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (24667-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Various lysates were subjected to SDS PAGE followed by western blot with 20024-1-AP (TBC1D4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20024-1-AP (TBC1D4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TBC1D4 antibody (20024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TBC1D4 (20024-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
TBC1D4 Monoclonal antibodies (1E2H2) (3 total products)
