Non-treated THP-1 cells and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.
Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68102-1-Ig (NLRP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68102-1-Ig (NLRP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).