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Mouse CD80/B7-1 Recombinant antibody, PBS Only (Capture/Detector)

CD80/B7-1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241371E12

Cat no : 84137-4-PBS

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01051-4

    Sandwich ELISA standard curve of MP01051-4

    Sandwich ELISA standard curve of MP01051-4, Mouse CD80/B7-1 Recombinant Matched Antibody Pair - PBS only. 84137-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0840. 84137-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Sample test of MP01051-4

    Sample test of MP01051-4

    The mean CD80/B7-1 concentration was determined to be 33,454.1 pg/mL in C2C12 cell extract based on a 3.2 mg/mL extract load.

  • Sample test of MP01051-4

    Sample test of MP01051-4

    Mouse spleen tissue was cultured in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD80/B7-1, and measured 10.44 pg/mL.

  • Sample test of MP01051-4

    Sample test of MP01051-4

    Serum of five mice was measured. The mouse CD80/B7-1 concentration of detected samples was determined to be 1,731.45 pg/mL with a range of 1,416.7 - 2,090.4 ng/mL.

  • Cytometric bead array standard curve of MP01051-1

    Cytometric bead array standard curve of MP01051-1

    Cytometric bead array standard curve of MP01051-1, MOUSE CD80 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84137-2-PBS. Detection antibody: 84137-4-PBS. Standard: Eg0840. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01051-2

    Cytometric bead array standard curve of MP01051-2

    Cytometric bead array standard curve of MP01051-2, MOUSE CD80 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84137-4-PBS. Detection antibody: 84137-1-PBS. Standard: Eg0840. Range: 0.313-40 ng/mL.

Product Information

84137-4-PBS targets CD80/B7-1 as part of a matched antibody pair:

MP01051-1: 84137-2-PBS capture and 84137-4-PBS detection (validated in Cytometric bead array)

MP01051-2: 84137-4-PBS capture and 84137-1-PBS detection (validated in Cytometric bead array)

MP01051-4: 84137-6-PBS capture and 84137-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD80 antigen
Calculated Molecular Weight35 kDa
GenBank Accession NumberNM_009855.2
Gene Symbol Cd80
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.