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Mouse CD80/B7-1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP01051-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01051-4, Mouse CD80/B7-1 Recombinant Matched Antibody Pair - PBS only. 84137-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0840. 84137-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

    Sandwich ELISA standard curve of MP01051-4

    Sandwich ELISA standard curve of MP01051-4, Mouse CD80/B7-1 Recombinant Matched Antibody Pair - PBS only. 84137-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0840. 84137-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Serum of five mice was measured. The mouse CD80/B7-1 concentration of detected samples was determined to be 1,731.45 pg/mL with a range of 1,416.7 - 2,090.4 ng/mL.

    Sample test of MP01051-4

    Serum of five mice was measured. The mouse CD80/B7-1 concentration of detected samples was determined to be 1,731.45 pg/mL with a range of 1,416.7 - 2,090.4 ng/mL.

  • The mean CD80/B7-1 concentration was determined to be 33,454.1 pg/mL in C2C12 cell extract based on a 3.2 mg/mL extract load.

    Sample test of MP01051-4

    The mean CD80/B7-1 concentration was determined to be 33,454.1 pg/mL in C2C12 cell extract based on a 3.2 mg/mL extract load.

  • Mouse spleen tissue was cultured in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD80/B7-1, and measured 10.44 pg/mL.

    Sample test of MP01051-4

    Mouse spleen tissue was cultured in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD80/B7-1, and measured 10.44 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01051-4 targets CD80/B7-1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse CD80 Recombinant antibody, PBS Only (Capture) 84137-6-PBS (241371A6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse CD80/B7-1 Recombinant antibody, PBS Only (Capture/Detector) 84137-4-PBS (241371E12). 100 μg. Concentration 1 mgl/ml.

Alternative CD80/B7-1 matched antibody pairs: MP00284-1, MP01051-1, MP01051-2, MP01051-3, MP50098-1

Matched pair protein standard: Eg0840

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84137-6-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD80 antigen
Synonyms Ly 53, CD80 antigen, Cd28l, B7.1, B7 1
GenBank accession numberNM_009855.2
Gene symbol Cd80
UniprotQ00609-1
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84137-4-PBS
Tested Reactivity mouse
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD80 antigen
Synonyms Cd80, CD80 antigen, Cd28l, B7.1, B7 1
GenBank accession numberNM_009855.2
Gene symbol Cd80
UniprotQ00609-1
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.