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Neurofibromin Recombinant antibody, PBS Only (Capture)

Neurofibromin Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240540F2

Cat no : 83620-5-PBS

Synonyms

NF1, NF-1, NF 1, neurofibromin 1, 240540F2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    WB analysis using 83620-5-RR (same clone as 83620-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83620-5-RR (Neurofibromin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    FC experiment of U2OS using 83620-5-RR (same clone as 83620-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Neurofibromin 1 Recombinant antibody (83620-5-RR)

    FC experiment of HeLa using 83620-5-RR (same clone as 83620-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Neurofibromin 1 Recombinant antibody (83620-5-RR, Clone:240540F2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83620-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00570-2

    Cytometric bead array standard curve of MP00570-2

    Cytometric bead array standard curve of MP00570-2, Neurofibromin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83620-5-PBS. Detection antibody: 83620-4-PBS. Standard: Ag25799. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83620-5-RR

    Affinity and Kinetic Characterization of 83620-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83620-5-RR against Human Neurofibromin 1 were performed. The affinity constant is below 1 pM.

Product Information

83620-5-PBS targets Neurofibromin as part of a matched antibody pair:

MP00570-2: 83620-5-PBS capture and 83620-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Neurofibromin fusion protein Ag25799
Full Name neurofibromin 1
Calculated Molecular Weight 319 kDa
Observed Molecular Weight250-280 kDa
GenBank Accession NumberM60915
Gene Symbol Neurofibromin 1
Gene ID (NCBI) 4763
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.