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Mouse CD19 Recombinant antibody, PBS Only (Capture/Detector)

CD19 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240135A3

Cat no : 83254-1-PBS

Synonyms

CD19 antigen, 240135A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD19 Recombinant antibody (83254-1-RR)

    WB analysis using 83254-1-RR (same clone as 83254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83254-1-RR (CD19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of rat spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD19 Recombinant antibody (83254-1-RR)

    IHC staining of mouse spleen using 83254-1-RR (same clone as 83254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83254-1-RR (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83254-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1

    Cytometric bead array standard curve of MP00253-1, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-2-PBS. Detection antibody: 83254-1-PBS. Standard: Eg0907. Range: 2.5-80 ng/mL.

  • Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3

    Cytometric bead array standard curve of MP00253-3, Mouse CD19 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83254-1-PBS. Detection antibody: 83254-4-PBS. Standard: Eg0907. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83254-1-RR

    Affinity and Kinetic Characterization of 83254-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83254-1-RR against Mouse CD19 were performed. The affinity constant is 0.303 nM.

Product Information

83254-1-PBS targets CD19 as part of a matched antibody pair:

MP00253-1: 83254-2-PBS capture and 83254-1-PBS detection (validated in Cytometric bead array)

MP00253-3: 83254-1-PBS capture and 83254-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name CD19 antigen
Calculated Molecular Weight60 kDa
Observed Molecular Weight62-70 kDa
GenBank Accession NumberNM_009844.2
Gene Symbol Cd19
Gene ID (NCBI) 12478
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD19 is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).