Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

Recombinant human Wnt3A (HZ-1296) induces dose-dependent alkaline phosphatase production in MC3T3 E1 mouse preosteoblast cells using pNPP as chromogenic substrate. MC3T3 E1 cells were treated with increasing concentrations of recombinant Wnt3A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-17 ng/mL.

Recombinant human Wnt3A (HZ-1296) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

Proteintech Wnt3A (HZ-1296) demonstrates a 3-fold lower EC50 and greater induction of luciferase production than a leading competitor. Recombinant human Wnt3A induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

Purity of recombinant human Wnt3A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

GMP Recombinant human GMP Wnt3A (HZ-1296-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of GMP recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 25-500 ng/mL

GMP Recombinant human GMP Wnt3A (HZ-1296-GMP) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of GMP recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 25-500 ng/mL.

mouse heart tissue were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

HUVEC cells were subjected to SDS PAGE followed by western blot with 55184-1-AP (WNT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55184-1-AP (WNT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26744-1-AP (WNT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10605-1-AP (WNT7A/B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP result of anti-WNT7A (IP:10605-1-AP, 4ug; Detection:10605-1-AP 1:4000) with mouse kidney tissue lysate 2280 ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (10605-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 29793-1-AP (WNT5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT5A antibody (29793-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC2051 (WNT10B IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using KHC2051 (WNT10B IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 27177-1-AP (WNT7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WNT7A antibody (27177-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

WNT5A fusion protein Ag31507 SDS-PAGE

WNT7A fusion protein Ag26085 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 27935-1-AP (WNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using WNT1 antibody (27935-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human WNT1 (27935-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 40x lens).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14371-1-AP (WNT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14371-1-AP (WNT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using WNT4 antibody (14371-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24201-1-AP (WNT6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24201-1-AP (WNT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 26238-1-AP (WNT10A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 26238-1-AP (WNT10A Antibody) at dilution of 1:100 (under 40x lens).