IP Result of anti-alpha Tubulin (IP: 66031-1-Ig, 5ug; Detection:11224-1-AP 1:1000) with HeLa cells lysate 2800ug. SA00001-2 (HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H+L) ) as the secondary antibody.
Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. SA00001-1 (HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) as secondary antibody.
Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and SA00007-2 (Rhodamine (TRITC)–conjugated Goat Anti-Rabbit IgG(H+L) (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-4 (HRP-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with Biotin-60293 (OPTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SA00001-0 (HRP-conjugated Streptavidin) as secondary antibody, at dilution of 1:3000 (lane 1 and 3) or 1:6000 (lane 2 and 4).
IgG proteins of various species were subjected to SDS PAGE followed by western blot with SA00001-3 (HRP-conjugated Affinipure Donkey Anti-Goat IgG(H+L) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Alpaca anti-human IgG/anti-rabbit IgG nano secondaries (5 total products)
