Jurkat cells (25 μg/lane) were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PARP1 (13371-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Anti-Fas treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.
IP result of anti-PARP1 (IP:13371-1-AP, 4ug; Detection:13371-1-AP 1:600) with K-562 cells lysate 5000ug.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13371-1-AP (PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 13371-1-AP (PARP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PARP1 antibody (13371-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
