Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 20687-1-AP (NOTCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20687-1-AP (NOTCH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-NOTCH1(IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:600) with HEK-293 cells lysate 1480 ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOTCH1 antibody (20687-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

IP result of anti-NOTCH1(IP:20687-1-AP, 4ug; Detection:20687-1-AP 1:500) with HepG2 cells lysate 1360 ug.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10062-2-AP (Notch1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10062-2-AP (Notch1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10062-2-AP (Notch1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using KHC1061 (NOTCH1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1061 (NOTCH1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using KHC1061 (NOTCH1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using KHC1061 (NOTCH1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC1061 (NOTCH1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1061 (NOTCH1 IHC Kit).

Notch1 fusion protein Ag0107 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-NOTCH3 (IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NOTCH3 (55114-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of CHAC1 antibody (15207-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHAC1 transfected HeLa cells.

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 10x lens).

U-87 MG cells were subjected to SDS PAGE followed by western blot with 28580-1-AP (NOTCH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with U-87 MG cells lysate 1960 ug.

IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with HEK-293 cells lysate 1575 ug.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human NOTCH3 (CL488-55114) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).