WB result of N-cadherin antibody (22018-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.
C2C12 (N-cadherin+), HeLa (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP Result of anti-N-cadherin (IP:22018-1-AP, 3ug; Detection:22018-1-AP 1:1500) with mouse brain tissue lysate 7000ug.
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (22018-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (22018-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 SH-SY5Y cells were stained with 0.2ug N-cadherin antibody (22018-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
