Various lysates were subjected to SDS PAGE followed by western blot with 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 20253-1-AP (IGF2R-Specific Antibody) at dilution of 1:50.
IP Result of anti-IGF2R-Specific (IP:20253-1-AP, 4ug; Detection:20253-1-AP 1:500) with HepG2 cells lysate 3480ug.
Immunofluorescent analysis of HepG2 cells using 20253-1-AP (IGF2R-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HepG2 cells were stained with .2ug IGF2R-Specific antibody (20253-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.
