Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13573-1-AP (TGN46 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidin stains F-actin (red).
IF result of TGN46 antibody (1:50) with Human Fibroblast (primary cells) By Dr. Neeraj Tiwar, Rothman Lab, Yale School of Medicine. Fixed with 4% PFA 10 min.
Various lysates were subjected to SDS PAGE followed by western blot with 13573-1-AP (TGN46 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP Result of anti-TGN46 (IP:13573-1-AP, 3ug; Detection:13573-1-AP 1:300) with HeLa cells lysate 2400ug.