Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using GLUT1 antibody (66290-1-Ig, Clone: 2A5A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (66290-1-Ig, Clone:2A5A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.