Various lysates were subjected to SDS PAGE followed by western blot with 11245-1-AP (PDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11245-1-AP (PDI antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PDI (11245-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP Result of anti-PDI (IP:11245-1-AP, 3ug; Detection:11245-1-AP 1:300) with mouse liver tissue lysate 4000ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDI antibody (11245-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).