Adult mouse small intestine was co-stained with desmin (CL488-16520, in cyan), E-cadherin (20874-1-AP, in red), and PCNA (60097-1-Ig, in green). Desmin marks the stromal cells and smooth muscle, E-cadherin marks the gut epithelial cells, and PCNA marks proliferating cells. The image was created in paid partnership with @Immunofluorescence on Instagram.

Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:100.

E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmin (CL488-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®647 Desmin antibody (CL647-16520) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®647 Desmin antibody (CL647-16520) at dilution of 1:200.

1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®647 Anti-Human Desmin (CL647-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Desmin (CL594-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 16520-1-AP (Desmin antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16520-1-AP (Desmin antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16520-1-AP (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Desmin antibody (16520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-Desmin (IP:16520-1-AP, 4ug; Detection:16520-1-AP 1:3000) with mouse heart tissue lysate 8000ug.

1X10^6 C2C12 cell were stained with 0.2ug Desmin antibody (16520-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Desmin antibody (16520-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue).

Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 Desmin antibody (CL594-67793, Clone: 2B1A11 ) at dilution of 1:200. DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

mouse heart tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).