Cell Line：HeLa cell line <br>Medium：DMEM+10%FBS <br>Culture Conditions：37℃，5%CO2，add CCK-8 for 2h, 450nm R2=0.9953<br>

The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

Staurosporine treated HeLa cells for 4.5 h.

The cell population was analyzed in THE SSC/FSC diagram:<br>FITC-A was selected on the X axis to denote CL488-AnnexinV. The Y-axis is represented by PE-A.<br><br>The meanings of the regions in the figure above:<br>Q1-UL (CL488-AnnexinV)-/PI+, cells in this region are necrotic. There may also be a small number of late apoptotic cells in it, even those with mechanical damage.<br>Q1-UR :(cl488-annexinv)+/PI+, cells in this region are late apoptotic cells.<br>Q1-LR :(cl488-annexinv)+/PI-, cells in this region are early apoptotic cells.<br>Q1-LL :(cl488-annexinv)-/PI-, cells in this region are living cells.<br><br>The apoptosis rate was usually calculated using Q1-UR+Q1-LR, late apoptosis + early apoptosis group (i.e., all AnnexinV-positive groups).

Green: Staining with CL488-Annexin V for apooptotic cells or early apoptotic cells;<br>Red: Staining with PI for dead cells;<br>Yellow: double staining with CL488-Annexin V and PI for necrotic cell or late apoptotic cells.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using the CoraLite® Plus 488-conjugated Phalloidin antibody, CL488-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

NIH3T3 mouse fibroblasts. 1:50 dilution of CoraLite® Plus 488-Phalloidin. Nuclei are stained with Hoechst 33342. Scale bar is 10 um. Images taken by Katarzyna Szymanksa-DeWijs from Hannover Medical School.

The above experimental results are based on Jurkat cells cultured for three days to perform a flow cytometry experiment to detect the apoptotic cells in the cell sample.

Staurosporine treated HeLa cells for 4 h.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using the CoraLite®594-conjugated Phalloidin antibody, CL594-Phalloidin, at dilution of 1:400. The nuclei were counterstained with DAPI.

Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye Blue 516 Viability Dye. Viable gate is indicated.

Mouse thymocytes cells were heat killed at 65℃ for 10 minutes and then mixed with live mouse thymocytes cells. Cells were then stained with Phantom Dye Red 710 Viability Dye. Viable gate is indicated.