MCF-7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of RAW 264.7 cells using 22601-1-AP (VEGFC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Standard curve of Human VEGF-C ELISA Kit KE00252.

Purity of recombinant human VEGF-C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human VEGF-C (HZ-1336) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF-C for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-14 ng/mL.
 

Immunohistochemical analysis of paraffin-embedded human lung tissue slide using KHC1323 (VEGFC IHC Kit).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC1323 (VEGFC IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using KHC1323 (VEGFC IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using KHC1323 (VEGFC IHC Kit).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67116-1-PBS in a different storage buffer formulation.