Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using STIM1 antibody (11565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human STIM1 (11565-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 555 Anti-Human STIM1 (CL555-11565) (red), or 0.4 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human STIM1 (CL647-11565) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81156-1-PBS in a different storage buffer formulation.

STIM1 fusion protein Ag17914 SDS-PAGE

K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66189-1-PBS in a different storage buffer formulation.