Various lysates were subjected to SDS PAGE followed by western blot with 11565-1-AP (STIM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11565-1-AP (STIM1 antibody) at dilution of 1:50 (under 40x lens).

1X10^6 HepG2 cells were stained with .2ug STIM1 antibody (11565-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human STIM1 (CL647-11565) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 81156-1-RR (STIM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

STIM1 fusion protein Ag17914 SDS-PAGE

K-562 cells were subjected to SDS PAGE followed by western blot with 66189-1-Ig (STIM1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66189-1-Ig (STIM1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).