Various lysates were subjected to SDS PAGE followed by western blot with 14123-1-AP (RPS3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14123-1-AP (RPS3A antibody) at dilution of 1:100 (under 40x lens).
IP Result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:500) with HepG2 cells lysate 3600ug.
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14123-1-AP (RPS3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 HepG2 cells were stained with 0.2ug RPS3A antibody (14123-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.