Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15340-1-AP (RPL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
MCF7 cells were subjected to SDS PAGE followed by western blot with 15340-1-AP (RPL7A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells, using RPL7A antibody 15340-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-RPL7A (IP:15340-1-AP, 4ug; Detection:15340-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.