Various lysates were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PSMD7 (IP:16034-1-AP, 3ug; Detection:16034-1-AP 1:500) with K-562 cells lysate 2400ug.
1X10^6 HepG2 cells were stained with 0.2ug PSMD7 antibody (16034-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
