A431 cells were subjected to SDS PAGE followed by western blot with 13773-1-AP (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Result of anti-P-cadherin (IP:13773-1-AP, 4ug; Detection:13773-1-AP 1:300) with A431 cells lysate 3040ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human P-cadherin (13773-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).