Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

MCF-7 cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 28411-1-AP (ORAI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28411-1-AP (ORAI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66223-1-Ig (ORAI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ORAI1 antibody (66223-1-Ig, Clone: 3C12F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66223-1-PBS in a different storage buffer formulation.

A375 cells were subjected to SDS PAGE followed by western blot with 28637-1-AP (ORAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

A549 cells were subjected to SDS PAGE followed by western blot with 28637-1-AP (ORAI1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

human spleen tissue were subjected to SDS PAGE followed by western blot with 14443-1-AP (Orai1-Alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14443-1-AP (Orai1-Alpha antibody at dilution of 1:50 (under 40x lens).

ORAI1 fusion protein Ag29249 SDS-PAGE

ORAI1 fusion protein Ag29651 SDS-PAGE

ORAI1 fusion protein Ag29779 SDS-PAGE