Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Myosin Light Chain 2/MLC-2V antibody (10906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10906-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10906-1-AP (Myosin Light Chain 2 antibody at dilution of 1:200 (under 10x lens).
IP result of anti-Myosin Light Chain 2/MLC-2V (IP:10906-1-AP, 3ug; Detection:10906-1-AP 1:500) with mouse heart tissue lysate 6000ug.
Various lysates were subjected to SDS PAGE followed by western blot with 10906-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10906-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 10906-1-AP (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Myosin Light Chain 2/MLC-2V (10906-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
