MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP Result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10861-1-AP (MECP2 Antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of HepG2 cells using 10861-1-AP (MECP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (10861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.