Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 U-87 MG cells were surface stained with 0.8 ug Anti-Human F11R (26941-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were not fixed .
Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.