Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GOLGA2/GM130 (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens).