Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells.
IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug.
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CFLAR/FLIP antibody (10394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).