Various lysates were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human ALCAM (65223-1-Ig, Clone:3A6) and CL594-Goat anti-Mouse IgG at dilution 1:1000 (red) or unstained (blue). Cells were not fixed. Monocytes were gated.

1X10^6 PHA activated human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human ALCAM (AB-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human ALCAM (CR-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul Cardinal Red™ Anti-Human ALCAM (CR-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human ALCAM (CL405-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5ul CoraLite® Plus 405 Anti-Human ALCAM. Cells were not fixed. Monocytes were gated.

1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human ALCAM (CL488-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul CoraLite® Plus 488 Anti-Human ALCAM (CL488-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®488 ALCAM antibody (CL488-67768, Clone: 1F7D6 ) at dilution of 1:200.