Various lysates were subjected to SDS PAGE followed by western blot with 10427-2-AP (Calnexin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Calnexin antibody (10427-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was stained with CL594-Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10427-2-AP (Calnexin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1X10^6 HeLa cells were stained with 0.2ug Calnexin antibody (10427-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.