K-562 cells were subjected to SDS PAGE followed by western blot with 10743-1-AP (Centromere protein R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10743-1-AP (Centromere protein R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).