A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were stained with 0.2ug CD146/MCAM antibody (66153-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:100.

Various lysates were subjected to SDS PAGE followed by western blot with 17564-1-AP (CD146/MCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WB result of CD146/MCAM antibody (17564-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD146/MCAM transfected HepG2 cells.

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17564-1-AP (CD146/MCAM antibody at dilution of 1:600 (under 40x lens).

IP Result of anti-CD146/MCAM (IP:17564-1-AP, 4ug; Detection:17564-1-AP 1:1500) with HeLa cells lysate 4000ug.

Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (17564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HUVEC cells were stained with 0.2ug CD146/MCAM antibody (17564-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

1X10^6 HeLa cells were surface stained with 0.2 ug Anti-Human CD146 (65181-1-Ig, Clone:P1H12) and APC-conjugated Goat Anti-Mouse IgG at dilution 1:1000. Cells were not fixed.

1X10^6 HeLa cells were surface stained with 10.00 ul/test PE Anti-Human CD146 (PE-65181, Clone:P1H12) (red) or isotype control antibody (blue). Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 CD146/MCAM antibody (CL555-66153, Clone: 4D8A9 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 CD146/MCAM antibody (CL555-66153, Clone: 4D8A9 ) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD146/MCAM (CL594-66153, Clone:4D8A9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 A375 cells were surface stained with 5 ul FITC Plus Anti-Human CD146 (FITC-65181, Clone: P1H12) (red) or Mouse IgG1 Isotype Control. Cells were not fixed.

1X10^6 A375 cells were surface stained with 5 ul FITC Plus Anti-Human CD146 (FITC-65181, Clone: P1H12) or Mouse IgG1 Isotype Control. Cells were not fixed.

Standard curve of Human CD146/MCAM ELISA Kit KE00065.