Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. beta actin is not expressed in adult heart thus we don't recommend it for heart tissue detection.
A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP (ACTB antibody) at dilution of 1:500.
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (20536-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Beta Actin (20536-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.