Various lysates were subjected to SDS PAGE followed by western blot with 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB result of BCRP,ABCG2 antibody (27286-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCRP,ABCG2 transfected MCF-7 cells.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27286-1-AP (BCRP,ABCG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
