PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of PC-3 cells using 11264-1-AP (ATG12 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66665-1-Ig (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Untreated and chloroquine treated HEK-293 cells, untreated and chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

U-937 cells were subjected to SDS PAGE followed by western blot with 21997-1-AP (LAMP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-P62,SQSTM1(IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug.

IP result of anti-ATG5(IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1800 ug.

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3 antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 20986-1-AP (ULK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66665-1-Ig (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 26276-1-AP (ATG9A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Untreated and chloroquine treated HEK-293 cells, untreated and chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with 81004-1-RR (LC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Lysates of HepG2 cells treated with Chloroquine or not were subjected to SDS PAGE followed by western blot with 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 81803-1-RR (ATG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 29445-1-AP (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 80992-1-RR (ubiquitin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

U-937 cells were subjected to SDS PAGE followed by western blot with 21997-1-AP (LAMP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IP result of anti-P62,SQSTM1(IP:80294-1-RR, 4ug; Detection:80294-1-RR 1:500) with HEK-293 cells lysate 1640 ug.

IP result of anti-ATG5(IP:81803-1-RR, 4ug; Detection:81803-1-RR 1:2000) with HeLa cells lysate 1800 ug.

Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81004-1-RR (LC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80294-1-RR (P62,SQSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81803-1-RR (ATG5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29445-1-AP (ATG16L1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26276-1-AP (ATG9A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20°C Methanol) fixed Chloroquine treated HeLa cells using LC3 antibody (81004-1-RR, Clone: 5P12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HeLa cells using P62,SQSTM1 antibody (80294-1-RR, Clone: 1D17 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ATG16L1 antibody (29445-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ubiquitin antibody (80992-1-RR, Clone: 6H6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 ATG12 antibody (CL488-11264) at dilution of 1:200.

Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

IP result of anti-ATG7 (IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with HT-1080 cells lysate 3600 ug.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (10088-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.

1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LNCaP cells were subjected to SDS PAGE followed by western blot with 67341-1-Ig (ATG7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67341-1-Ig (ATG7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67341-1-Ig (ATG7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

WB result of ATG7 antibody (67341-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells. This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATG7 (67341-1-Ig, Clone:2B5A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67341-1-PBS in a different storage buffer formulation.