1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD45 (CL488-65087, Clone:30-F11) (red), or 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2) (blue). Cells were not fixed.