1X10^6 mouse splenocytes were surface stained with 0.2 ug CoraLite® Plus 647 Anti-Mouse CD38 (CL647-65059, Clone:90) or 0.2 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3), and 0.2 ug FITC Plus Anti-Mouse CD3ε (145-2C11) (FITC-65060, Clone: 145-2C11). Cells were not fixed.