Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using GLUT1 antibody (66290-1-Ig, Clone: 2A5A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 40x lens).

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (66290-1-Ig, Clone:2A5A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GLUT1 (CL488-66290, Clone:2A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 GLUT1 antibody (CL594-66290, Clone: 2A5A2 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite®594 GLUT1 antibody (CL594-66290, Clone: 2A5A2 ) at dilution of 1:200.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GLUT1 (CL594-66290, Clone:2A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using GLUT1 antibody (66290-1-Ig, Clone: 2A5A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (66290-1-Ig, Clone:2A5A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC0036 (GLUT1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0036 (GLUT1 IHC Kit)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0036 (GLUT1 IHC Kit). This view was captured from cancer adjacent region. Note that red blood cells are well stained.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0036 (GLUT1 IHC Kit)

HT-29 cell lysates (boiled or unboiled) were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (21829-1-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21829-1-AP (GLUT1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLUT1 antibody (21829-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

1x10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GLUT1 (CL488-21829)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).