uPAR/CD87 Recombinant antibody, PBS Only (Capture/Detector)

uPAR/CD87 Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230148B10

Cat no : 82953-2-PBS

Synonyms

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

---

Validation Data Gallery

• 

  WB analysis using 82953-2-RR (same clone as 82953-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00129-1

  Sandwich ELISA standard curve of MP00129-1, uPAR, PLAUR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-2-PBS. Detection antibody: 82953-3-PBS. Standard: Ag28056. Range: 125-8000 pg/mL.

• 

  Sandwich ELISA standard curve of MP00129-2

  Sandwich ELISA standard curve of MP00129-2, uPAR, PLAUR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-4-PBS. Detection antibody: 82953-2-PBS. Standard: Ag28056. Range: 125-8000 pg/mL.

• 

  IHC staining of mouse spleen using 82953-2-RR (same clone as 82953-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82953-2-RR (same clone as 82953-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR (same clone as 82953-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00129-2

  Cytometric bead array standard curve of MP00129-2, uPAR, PLAUR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82953-4-PBS. Detection antibody: 82953-2-PBS. Standard: Ag28056. Range: 1.25-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 82953-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-2-RR against Human uPAR, PLAUR were performed. The affinity constant is 0.527 nM.

---

Product Information

82953-2-PBS targets uPAR/CD87 as part of a matched antibody pair:

MP00129-1: 82953-2-PBS capture and 82953-3-PBS detection (validated in Sandwich ELISA)

MP00129-2: 82953-4-PBS capture and 82953-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35-50 kDa
• GenBank Accession Number: BC002788
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)