uPAR/CD87 Recombinant antibody, PBS Only

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  IHC staining of human ovary cancer using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82953-1-RR (same clone as 82953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  IF Staining of HT-1080 using 82953-1-RR (same clone as 82953-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using uPAR/CD87 antibody (82953-1-RR, Clone: 230148A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82953-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82953-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-1-RR against Human uPAR, PLAUR were performed. The affinity constant is 1.49 nM.

uPAR/CD87 {ptg:Brand} Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 82953-1-PBS

Clone No.230148A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

uPAR, PLAUR, PLAUR, 230148A1, CD87, MO3

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Product Information

82953-1-PBS targets uPAR/CD87 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC002788
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03405
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)