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uPAR, PLAUR Monoclonal antibody, PBS Only

uPAR, PLAUR Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A3E8

Cat no : 68727-1-PBS

Synonyms

CD87, MO3, PLAUR, U PAR, UPAR, URKR

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using uPAR, PLAUR Monoclonal antibody (68727-1-Ig)

    WB analysis of U2OS using 68727-1-Ig (same clone as 68727-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using uPAR, PLAUR Monoclonal antibody (68727-1-Ig)

    WB analysis using 68727-1-Ig (same clone as 68727-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68727-1-PBS targets uPAR, PLAUR in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen uPAR, PLAUR fusion protein Ag28056
Full Name PLAUR
Calculated Molecular Weight 37 kDa
Observed Molecular Weight35-50 kDa
GenBank Accession NumberBC002788
Gene Symbol PLAUR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)