uPAR/CD87 Monoclonal antibody, PBS Only

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  WB analysis of U2OS using 68727-1-Ig (same clone as 68727-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

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  WB analysis using 68727-1-Ig (same clone as 68727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung squamous cell cancer using 68727-1-Ig (same clone as 68727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung squamous cell cancer using 68727-1-Ig (same clone as 68727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68727-1-PBS in a different storage buffer formulation.

uPAR/CD87 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68727-1-PBS

Clone No.2A3E8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

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Product Information

68727-1-PBS targets uPAR/CD87 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: uPAR/CD87 fusion protein Ag28056
• Full Name: PLAUR
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 35-50 kDa
• GenBank Accession Number: BC002788
• Gene Symbol: uPAR
• Gene ID (NCBI): 5329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q03405
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)