Gamma Tubulin Polyclonal antibody

Gamma Tubulin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26195-1-AP

Synonyms

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

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Validation Data Gallery

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  WB analysis of mouse brain using 26195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 26195-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26195-1-AP (Gamma Tubulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: C2C12 cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26195-1-AP targets Gamma Tubulin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Gamma Tubulin fusion protein Ag23575
• Full Name: tubulin, gamma 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC000619
• Gene Symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• RRID: AB_2880420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.