transgelin/SM22 Monoclonal antibody, PBS Only

transgelin/SM22 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10C2

Cat no : 60213-1-PBS

Synonyms

22 kDa actin binding protein, DKFZp686P11128, Protein WS3 10, SM22, SM22 alpha, SM22-a, SM22alpha, SMCC, Smooth muscle protein 22 alpha, TAGLN, TAGLN1, transgelin, TRANSGELIN 1, transgelin/SM22, transgelin/SM22 alpha, WS3 10

---

Validation Data Gallery

• 

  WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat colon using 60213-1-Ig (same clone as 60213-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat stomach using 60213-1-Ig (same clone as 60213-1-PBS)

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60213-1-PBS targets transgelin/SM22 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, mouse, mouse samples.

• Tested Reactivity: human, rat, pig, mouse, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: transgelin/SM22 fusion protein Ag0764
• Full Name: transgelin
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 18+22 kDa
• GenBank Accession Number: BC004927
• Gene Symbol: TAGLN
• Gene ID (NCBI): 6876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.