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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-29 protein

Activity(Primary)

0.12-0.6 ng/mL

Specific Activity

minimally 8.00 x 105 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1156

Synonyms

Cytokine Zcyto21, IFN lambda 1, IFNL1, IFN-lambda 1, IL 29, IL29, IL-29, Interferon lambda 1, Interleukin 29, ZCYTO21

Validation Data Gallery

  • Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-29 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

    Recombinant human IL-29 (HZ-1156) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range is 0.12-0.6 ng/mL.

Technical Specifications

GeneID 282618
Species Human
Expression HEK293
Activity(Primary) 0.12-0.6 ng/mL
Specific Activityminimally 8.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ8IU54
Molecular Mass 27 and 31 kDa reduced, 25 and 30 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Interleukin-29（IL-29, also known as IFN gamma1）is a member of the helical cytokine family and is a type III interferon. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses (PMID: 18547367;12469119;27637354)

Synonyms

Cytokine Zcyto21, IFN lambda 1, IFNL1, IFN-lambda 1, IL 29, IL29, IL-29, Interferon lambda 1, Interleukin 29, ZCYTO21

Publications

SpeciesTitle

Int J Mol Sci

Multifaceted Analysis of IL-23A- and/or EBI3-Including Cytokines Produced by Psoriatic Keratinocytes.

Authors - Kota Tachibana
View Article

Antiviral Res

Antiviral candidates against the hepatitis E virus (HEV) and their combinations inhibit HEV growth in in vitro.

Authors - Takashi Nishiyama
View Article
human

Cell Rep

Critical role of Syk-dependent STAT1 activation in innate antiviral immunity.

Authors - Shasha Liu
View Article
human

J Virol Methods

Screening of novel drugs for inhibiting hepatitis E virus replication.

Authors - Takashi Nishiyama
View Article