HumanKine® recombinant human R-Spondin-1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

4-20 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Publications

3

Cat no : HZ-1328

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

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Validation Data Gallery

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  Purity of recombinant human R-Spondin-1 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human R-Spondin-1 (HZ-1328) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant R-Spondin-1 for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model.

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Technical Specifications

• GeneID: 284654
• Species: Human
• Expression: HEK293
• Activity(Primary): 4-20 ng/mL
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: 2QMKA7
• Molecular Mass: 36-50 kDa reduced and non-reduced, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

R-Spondin 1 is an approximately 40kDA, glycosylated protein that serves as a major agonist of the Wnt signaling pathway. It regulates the turnover of the LRP6 co-receptor by preventing its internalization by DKK-1. R-Spondin 1 plays several roles in embryonic development including sex phenotype reversal, female sex determination, and ovarian differentiation. It also promotes stem cell turnover in the mammary glands, intestine, colon, and kidneys. Increased R-Spondin 1 expression has been linked to growth and migration of ovarian cancer cells as well as decreased sensitivity of gliomas to radiation-based therapies (PMID: 17804805, 22439850, 32749219, 30572097).

Synonyms

Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Publications

Species	Title
mouse	Nat Commun; Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells; Authors - Yang Sun
human	Mol Neurobiol; Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis; Authors - Yipeng Zhao
human	Nat Commun; KAT8-mediated H4K16ac is essential for sustaining trophoblast self-renewal and proliferation via regulating CDX2; Authors - Shilei Bi