• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human R-Spondin-I protein


4-20 ng/mL EC50

Species Reactivity




Cat no : HZ-1328


Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1

Technical Specifications

GeneID 284654
Species Human
Expression HEK293
Activity(Primary) 4-20 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession Number2QMKA7
Molecular Mass 40 kDa, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).


R-Spondin 1 is an approximately 40kDA, glycosylated protein that serves as a major agonist of the Wnt signaling pathway. It regulates the turnover of the LRP6 co-receptor by preventing its internalization by DKK-1. R-Spondin 1 plays several roles in embryonic development including sex phenotype reversal, female sex determination, and ovarian differentiation. It also promotes stem cell turnover in the mammary glands, intestine, colon, and kidneys. Increased R-Spondin 1 expression has been linked to growth and migration of ovarian cancer cells as well as decreased sensitivity of gliomas to radiation-based therapies (PMID: 17804805, 22439850, 32749219, 30572097).


Roof-plate specific spondin-1, Critsin3, RSPO1,HRspo1



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Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells

Authors - Yang Sun