HumanKine® recombinant human IL-5 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.2-0.9 ng/mL

Specific Activity

minimally 5.0 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1324

Synonyms

IL5, IL 5, IL-5T-cell replacing factor, interleukin 5, interleukin-5, EDF, Eo-CSF, Eosinophil differentiation factor, BCDF mu, B-cell differentiation factor I, BCGFII, TRF, TRFB cell differentiation factor

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Validation Data Gallery

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  Purity of recombinant human IL-5 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human IL-5 (HZ-1324) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-5 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.2-0.9 ng/mL.

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Technical Specifications

• GeneID: 3567
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.2-0.9 ng/mL
• Specific Activity: minimally 5.0 x 10^6 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P05113
• Molecular Mass: 19 to 21 kDa reduced, 35 to 40 kDa non-reduced, disulfide linked homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity (PMID: 21986312, 22174445, 19819937, 16895557)

Synonyms

IL5, IL 5, IL-5T-cell replacing factor, interleukin 5, interleukin-5, EDF, Eo-CSF, Eosinophil differentiation factor, BCDF mu, B-cell differentiation factor I, BCGFII, TRF, TRFB cell differentiation factor