HumanKine® recombinant human IL-22 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.045-0.225 ng/mL EC50

Species Reactivity

human

Purity

> 95%

Publications

1

Cat no : HZ-1325

Synonyms

IL-TIF, cytokine ZCYTO18

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Validation Data Gallery

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  Purity of recombinant human IL-22 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IL-22 (Cat no: HZ-1325) induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

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  Proteintech IL-22 (Cat no: HZ-1325) demonstrates equivalent induction of IL-10 release and EC50 compared to a leading competitor. Recombinant human IL-22 induces dose-dependent release of IL-10 in the Colo 205 human cell line. Colo 205 cells were treated with increasing concentration of recombinant IL-22 for 24 hours before supernatant collection. The supernatant was tested for IL-10 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.045-0.225 ng/mL.

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Technical Specifications

• GeneID: 50616
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.045-0.225 ng/mL EC50
• Purity: > 95%
• Endotoxin: <1.0 EU/μg
• Accession Number: Q9GZX6
• Molecular Mass: 25-40 kDa reduced and non-reduced, dimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-22 is a 6-α-helix, bundle cytokine that is a member of the IL-10 family of cytokines. It is primarily produced by innate lymphoid cells (ILCS) and multiple T cell subsets including αβT, γδT, and NKT cells. Its production is regulated upstream by several different cytokines including IL-23, IL-1β, and IL-7. IL-22 binds with the IL-22 receptor, expressed on stromal and epithelial cells in a wide range of tissues, but it can also bind in circulation with the IL-22 binding protein (IL-22BP), which functions as a negative regulator. Binding with the receptor triggers several downstream pathways resulting in pro-inflammatory responses, increased mucus production, as well as increased production of anti-bacterial and anti-fungal proteins. IL-22 also plays a role in promoting wound healing and tissue regeneration in response to injury. Overexpression of IL-22 has been linked to several cancers, most notably colon cancer (PMID: 25706098, 33042126).

Synonyms

IL-TIF, cytokine ZCYTO18

Publications

Species	Title
human	Skin Res Technol; Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis; Authors - Delu Che