HumanKine® recombinant human IL-15 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

0.01-0.15 ng/mL in reporter assay

Specific Activity

minimally 3.50 x 10⁷ IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1337-GMP

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15, IL-15

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Validation Data Gallery

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  Purity of recombinant human IL-15 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IL-15 (Cat no: HZ-1337) stimulates dose-dependent induction of alkaline phosphatase production in a HEK293 reporter cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 0.01-0.15 ng/mL EC50.

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Technical Specifications

• GeneID: 3600
• Species: Human
• Expression: HEK293
• EC50: 0.01-0.15 ng/mL in reporter assay
• Activity(Secondary): 0.07-0.37 ng/mL for NK-92 bioassay.
• Specific Activity: minimally 3.50 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <0.1 EU/µg
• Accession Number: P40933
• Molecular Mass: 12 to 24 kDa reduced, 11 to 23 kDa non-reduced, monomer, glycosylated
• Formulation: 50 mM Acetate pH 4.0 + 150mM NaCl
• Species Reactivity: human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common γ chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)

Synonyms

Interleukin 15, IL15, IL-15MGC9721, Interleukin-15, IL-15