HumanKine® recombinant human FGF-19 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

50-300 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

1

Cat no : HZ-1330

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

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Validation Data Gallery

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  Purity of recombinant human FGF-19 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human FGF-19 (HZ-1330) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.02% FBS during treatment with increasing concentrations of recombinant human FGF-19 for 72hrs in defined medium. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 50-300 ng/mL.

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  HepG2 cells were serum starved overnight, and treated with FGF-19 (HZ-1330) for 30 min at indicated concentrations. Cells were harvested and lysed using RIPA buffer with inhibitors. Western blot was performed with Proteintech antibodies, phospho-ERK1/2 (Thr202/Tyr204) Polyclonal antibody (28733-1-AP) and ERK1/2 Polyclonal antibody (11257-1-AP).

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Technical Specifications

• GeneID: 9965
• Species: Human
• Expression: HEK293
• Activity(Primary): 50-300 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: O95750
• Molecular Mass: 23 kDa reduced, 22 kDa non-reduced, monomer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

FGF-19 is a member of the endocrine subfamily of FGFs. It primarily binds and activates signaling pathways downstream of FGFR4 in the liver. Through downregulation of the CYP7A1 enzyme, FGF-19 can prevent overproduction of bile salts in the liver and contribute towards their homeostasis. It is also a regulator of glucose and lipid metabolism alongside FGF-21. FGF-19 can additionally stimulate hepatocyte proliferation and has been correlated with the progression of hepatocellular carcinoma when its signaling levels are increased. (PMID: 30191047, 21437243, 20660733).

Synonyms

FGF-19, FGF19, FGFJ, Fibroblast Growth Factor-19

Publications

Species	Title
mouse	Cell Metab; Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis; Authors - Junliang Kuang